3 articles published in JoVE
High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials Stefan H. Geyer1, Barbara Maurer-Gesek1, Lukas F. Reissig1, Wolfgang J. Weninger1 1Division of Anatomy, Center for Anatomy and Cell Biology & MIC, Medical University of Vienna We provide simple and robust protocols for processing biopsy material of various species, embryos of biomedical model organisms and samples of other organic tissues in order to permit digital volume data generation with the high-resolution episcopic microscopy method.
A Unified Methodological Framework for Vestibular Schwannoma Research Lukas D. Landegger*1,2,3, Jessica E. Sagers*1,4, Sonam Dilwali1,4, Takeshi Fujita1,2, Mehmet I. Sahin1,2, Konstantina M. Stankovic1,2,4 1Eaton Peabody Laboratories, Department of Otolaryngology, Massachusetts Eye and Ear, 2Department of Otolaryngology, Harvard Medical School, 3Department of Otolaryngology, Vienna General Hospital, Medical University of Vienna, 4Program in Speech and Hearing Bioscience and Technology, Harvard Medical School The goal of this protocol is to outline the collection and processing of human surgical samples for multiple downstream applications in vestibular schwannoma and Schwann cell research.
Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects Camillo Sherif*1,2,3, Javier Fandino*2,4, Salome Erhardt2,4, Antonio di Ieva5, Monika Killer6, Guenther Kleinpeter1, Serge Marbacher2,4 1Department of Neurosurgery, Hospital Rudolfstiftung, 2Department of Neurosurgery, Kantonsspital Aarau, 3Cerebrovascular Research Group, Department of Biomedical Research, Medical University of Vienna, 4Cerebrovascular Research Group, Department of Intensive Care Medicine, University of Berne, 5Centre for Anatomy and Cell Biology, Department of Systematic Anatomy, Medical University of Vienna, 6Department of Neurology, Paracelsus University Salzburg An optimized technique for the microsurgical creation of arterial bifurcation aneurysms mimicking bifurcation human cerebral aneurysms is described. A venous pouch is sutured into an artificially created true bifurcation of both common carotid arteries. Facilitated microsurgical techniques and aggressive postoperative anticoagulation and analgesia lead to minimized morbidity rates and high aneurysm patency rates.