2 articles published in JoVE
Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice Mathilde Beka1, Teresinha Leal1 1Louvain Center for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain Here, we present a protocol to measure nasal potential difference in mice. The test quantifies the function of transmembrane ion transporters such as the cystic fibrosis transmembrane conductance regulator and the epithelial sodium channel. It is valuable to evaluate the efficacy of novel therapies for cystic fibrosis.
Expired CO2 Measurement in Intubated or Spontaneously Breathing Patients from the Emergency Department Franck Verschuren1, Maidei Gugu Kabayadondo1, Frédéric Thys1 1Acute Medicine Department Accidents and Emergency Unit, Universit Catholique de Louvain Cliniques Universitaires Saint-Luc This video describes how to perform CO2 measurement in intubated as well as spontaneously breathing patients. The main clinical indications refer to emergency situations: (1) verifying adequate positioning of an endotracheal tube; (2) achieving normocapnia in trauma patients; (3) monitoring ventilation in the case of procedural sedation.