2 articles published in JoVE
PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator Julie Bolcaen1, Benedicte Descamps2, Tom Boterberg3, Christian Vanhove2, Ingeborg Goethals1 1Department of Nuclear Medicine, Ghent University Hospital, 2IBiTech-MEDISIP, Department of Electronics and Information Systems, Ghent University, 3Department of Radiation Oncology, Ghent University Hospital In the past, small animal irradiation was usually performed without the ability to target a well-delineated tumor volume. The goal was to mimic the treatment of human glioblastoma in rats. Using a small animal irradiation platform, we performed MRI-guided 3D conformal irradiation with PET-based sub-volume boosting in a preclinical setting.
Cellular Redox Profiling Using High-content Microscopy Tom Sieprath1,2, Tobias Corne1,2, Joke Robijns1, Werner J. H. Koopman3, Winnok H. De Vos1,2 1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center This paper presents a high-content microscopy workflow for simultaneous quantification of intracellular ROS levels, as well as mitochondrial membrane potential and morphology – jointly referred to as mitochondrial morphofunction – in living adherent cells using the cell-permeant fluorescent reporter molecules 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) and tetramethylrhodamine methylester (TMRM).