2 articles published in JoVE
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry Suzan Wetzels*1,2,3, Mitchell Bijnen*1,2, Erwin Wijnands2,4, Erik A.L. Biessen2,4, Casper G. Schalkwijk1,2, Kristiaan Wouters1,2 1Department of Internal Medicine, MUMC, 2CARIM, MUMC, 3Department of Immunology and Biochemistry, Biomedical Research Institute, Hasselt University, 4Department of Pathology, MUMC This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.
Fundus Photography as a Convenient Tool to Study Microvascular Responses to Cardiovascular Disease Risk Factors in Epidemiological Studies Patrick De Boever1,2, Tijs Louwies1,2, Eline Provost1,2, Luc Int Panis1,3, Tim S. Nawrot2,4 1Environmental Risk and Health, Flemish Institute for Technological Research (VITO), 2Centre for Environmental Sciences, Hasselt University, 3Transportation Research Institute, Hasselt University, 4Department of Public Health, Occupational and Environmental Medicine, Leuven University Retinal image analysis is an unobtrusive procedure for visualizing the microcirculation. The impact of cardiovascular disease risk factors can result in changes of retinal vessel calibers. The procedures to acquire fundus images and the steps for calculating the vessel calibers are described.