Kantonsspital Aarau View Institution's Website 7 articles published in JoVE Neuroscience Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model Stefan Wanderer*1,2, Basil E. Grüter*1,2, Jeannine Kümin1,2, Gwendoline Boillat1,2, Sivani Sivanrupan2, Kristina Catalano1,2, Michael von Gunten3, Hans Rudolf Widmer4, Serge Marbacher1,2,5, Lukas Andereggen1,2,5 1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Institute of Pathology Laenggasse, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Faculty of Medicine, University of Bern We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls. Medicine Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit Gwendoline Boillat1,2, Tim Franssen2, Basil Grüter1,2, Stefan Wanderer1,2, Kristina Catalano2, Daniela Casoni3, Lukas Andereggen1,2, Serge Marbacher1,2 1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Experimental Surgery Facility, Department for Biomedical Research, Faculty of Medicine, University of Bern This protocol describes the steps for the creation of a rabbit model with two elastase-digested aneurysms with different hemodynamics (stump and bifurcation constellation). This allows the testing of novel endovascular devices in aneurysms with different angioarchitecture and hemodynamic conditions within a single animal. Neuroscience Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit Stefan Wanderer1,2, Claudia Waltenspuel2, Basil E. Grüter1,2, Fabio Strange1,2, Sivani Sivanrupan2, Luca Remonda3, Hans Rudolf Widmer4, Daniela Casoni5, Lukas Andereggen1,2, Javier Fandino1,2, Serge Marbacher1,2 1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Department for Biomedical Research, Faculty of Medicine, University of Bern Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits. Neuroscience Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models Fabio Strange1,2, Sivani Sivanrupan2, Basil E. Gruter1,2, Jeannine Rey2, Dominik Taeschler2, Javier Fandino1,2, Serge Marbacher1,2 1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research (DBMR), University of Bern We present a protocol to efficiently evaluate aneurysm perfusion and vessel patency of sidewall aneurysm in rats and rabbits, using fluorescein-based fluorescence video angiography (FVA). With a positive predictive value of 92.6%, it is a simple but very effective and economical method with no special equipment required. Neuroscience Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique Edin Nevzati1,2, Jeannine Rey2, Daniel Coluccia1,2, Donato D'Alonzo1,2, Basil Grüter1,2, Luca Remonda3, Javier Fandino1,2, Serge Marbacher1,2 1Department of Neurosurgery, Kantonsspital Aarau, 2Neuro Lab, Research Group for Experimental Neurosurgery and Neurocritical Care, Department of Intensive Care Medicine, University Hospital and University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau Reproducable experimental animal models are needed for the testing of novel embolization materials, which have been designed to treat endovascular occlusion of intracranial aneurysms (IA). The present study aims to develop a safe and standardized surgical technique for stent assisted embolization of saccular aneurysms in a rat animal model. Medicine The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects Lukas Andereggen3,4,5, Volker Neuschmelting1,6, Michael von Gunten7, Hans Rudolf Widmer5, Jukka Takala1, Stephan M. Jakob1, Javier Fandino1,2, Serge Marbacher1,2 1Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), 2Department of Neurosurgery, Kantonsspital Aarau, 3 The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation. Medicine Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects Camillo Sherif*1,2,3, Javier Fandino*2,4, Salome Erhardt2,4, Antonio di Ieva5, Monika Killer6, Guenther Kleinpeter1, Serge Marbacher2,4 1Department of Neurosurgery, Hospital Rudolfstiftung, 2Department of Neurosurgery, Kantonsspital Aarau, 3Cerebrovascular Research Group, Department of Biomedical Research, Medical University of Vienna, 4Cerebrovascular Research Group, Department of Intensive Care Medicine, University of Berne, 5Centre for Anatomy and Cell Biology, Department of Systematic Anatomy, Medical University of Vienna, 6Department of Neurology, Paracelsus University Salzburg An optimized technique for the microsurgical creation of arterial bifurcation aneurysms mimicking bifurcation human cerebral aneurysms is described. A venous pouch is sutured into an artificially created true bifurcation of both common carotid arteries. Facilitated microsurgical techniques and aggressive postoperative anticoagulation and analgesia lead to minimized morbidity rates and high aneurysm patency rates.
