2 articles published in JoVE
A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci Simon Blaser1,2,3, Hanspeter Diem4, Andreas von Felten4, Morgan Gueuning1, Michael Andreou5, Neil Boonham6,7, Jennifer Tomlinson6, Pie Müller2,3, Jürg Utzinger2,3, Beatrice Frey1, Jürg E. Frey1, Andreas Bühlmann8 1Department of Method Development and Analytics, Agroscope, 2Swiss Tropical and Public Health Institute, 3University of Basel, 4Swiss Federal Plant Protection Service, Federal Office for Agriculture, 5OptiGene Limited, 6Fera Science Limited, 7School of Natural and Environmental Sciences, Newcastle University, 8Department of Plants and Plant Products, Agroscope This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.
Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children Peiling Yap1,2, Thomas Fürst1,2, Ivan Müller1,2, Susi Kriemler1,2, Jürg Utzinger1,2, Peter Steinmann1,2 1Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland, 2University of Basel, Basel, Switzerland Chronic infection with soil-transmitted helminths (STHs) causes malabsorption, stunting, and wasting in the growing child. Hence, it is plausible that these infections also reduce the physical fitness of children. Here, we visualize two techniques for the diagnosis of STHs and the 20-meter shuttle run test for assessing children's physical fitness.