3 articles published in JoVE
Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications Stephanie Mauti1, Monique Léchenne2, Service Naïssengar3, Abdallah Traoré4, Vessaly Kallo5,6, Casimir Kouakou7, Emmanuel Couacy-Hymann7, Morgane Gourlaouen8, Céline Mbilo9,10, Pati Patient Pyana11, Enos Madaye3, Ibrahima Dicko4, Pascal Cozette1, Paola De Benedictis8, Hervé Bourhy1, Jakob Zinsstag9,10, Laurent Dacheux1 1Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, 2Environment and Sustainability Institute, University of Exeter, Penryn Campus, 3Institut de Recherche en Elevage pour le Développement, 4Laboratoire Central Vétérinaire, 5 We present a complete protocol for postmortem diagnosis of animal rabies under field conditions using a rapid immunochromatographic diagnostic test (RIDT), from brain biopsy sampling to final interpretation. We also describe further applications using the device for molecular analysis and viral genotyping.
A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci Simon Blaser1,2,3, Hanspeter Diem4, Andreas von Felten4, Morgan Gueuning1, Michael Andreou5, Neil Boonham6,7, Jennifer Tomlinson6, Pie Müller2,3, Jürg Utzinger2,3, Beatrice Frey1, Jürg E. Frey1, Andreas Bühlmann8 1Department of Method Development and Analytics, Agroscope, 2Swiss Tropical and Public Health Institute, 3University of Basel, 4Swiss Federal Plant Protection Service, Federal Office for Agriculture, 5OptiGene Limited, 6Fera Science Limited, 7School of Natural and Environmental Sciences, Newcastle University, 8Department of Plants and Plant Products, Agroscope This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.
Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children Peiling Yap1,2, Thomas Fürst1,2, Ivan Müller1,2, Susi Kriemler1,2, Jürg Utzinger1,2, Peter Steinmann1,2 1Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland, 2University of Basel, Basel, Switzerland Chronic infection with soil-transmitted helminths (STHs) causes malabsorption, stunting, and wasting in the growing child. Hence, it is plausible that these infections also reduce the physical fitness of children. Here, we visualize two techniques for the diagnosis of STHs and the 20-meter shuttle run test for assessing children's physical fitness.