3 articles published in JoVE
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis Patricia E. Nigg1,2, Jovan Pavlovic1 1Institute for Medical Virology, University of Zurich, 2Friedrich Miescher Institute for Biomedical Research This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.
Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment Marcus Leinweber*1,2, Pawel Zmarz*1,2, Peter Buchmann3, Paul Argast1, Mark Hübener2, Tobias Bonhoeffer2, Georg B. Keller1,2 1Friedrich Miescher Institute for Biomedical Research, 2Max Planck Institute of Neurobiology, 3Department of Biosystems Science and Engineering, ETH Zurich Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.
Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression Marta Gomez-Martinez1, Debora Schmitz2, Alexander Hergovich1 1UCL Cancer Institute, 2Friedrich Miescher Institute for Biomedical Research A rapid and simple way to generate human cell lines with inducible and reversible cDNA overexpression or shRNA-mediated knock-down of the gene of interest. This method enables researchers to reliably and highly reproducibly manipulate cell lines that are difficult to alter by transient transfection methods or conventional knockdown/knockout strategies.