2 articles published in JoVE
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements Siamak Djafarzadeh1, Stephan Mathias Jakob1 1Department of Intensive Care Medicine, Inselspital, Bern University Hospital Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays Stefan Hahnewald1,2, Marta Roccio1,2, Anne Tscherter3, Jürg Streit3, Ranjeeta Ambett1,2, Pascal Senn1,2,4 1Department of Otorhinolaryngology, Inselspital and University of Bern, 2Department of Clinical Research, Inselspital and University of Bern, 3Department of Physiology, University of Bern, 4Department of Clinical Neurosciences, Service ORL & HNS, University Hospital Geneva We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.