University Hospital and University of Bern

7 articles published in JoVE

• Neuroscience

  
  Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit Stefan Wanderer^1,2, Claudia Waltenspuel², Basil E. Grüter^1,2, Fabio Strange^1,2, Sivani Sivanrupan², Luca Remonda³, Hans Rudolf Widmer⁴, Daniela Casoni⁵, Lukas Andereggen^1,2, Javier Fandino^1,2, Serge Marbacher^{1,2 1}Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Department for Biomedical Research, Faculty of Medicine, University of Bern Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.

• Medicine

  
  Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy Sara Nagy^1,2, Simone Schmidt^1,2, Patricia Hafner^1,3, Andrea Klein^1,4,5, Daniela Rubino-Nacht¹, Vanya Gocheva¹, Oliver Bieri⁶, Carole Vuillerot⁷, Ulrike Bonati^1,2, Dirk Fischer^{1,2,3 1}Division of Pediatric Neurology, University Children's Hospital Basel (UKBB), University of Basel, ²Department of Neurology, University Hospital Basel, University of Basel, ³Division of Neurology, University Clinic of Medicine, Cantonal Hospital Baselland, Bruderholz, ⁴Division of Pediatric Neurology, CHUV, ⁵Division of Neuropaediatrics, Inselspital, University Children's Hospital Bern, ⁶Department of Radiology, Division of Radiological Physics, University Hospital Basel, University of Basel, ⁷Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, L'Escale, Service de Médecine Physique et de Réadaptation Pédiatrique The aim of this study is to present the most reliable clinical outcome measures and their correlations with quantitative muscle MRI in ambulant patients with Duchenne muscular dystrophy.

• Biology

  
  Cystometric and External Urethral Sphincter Measurements in Awake Rats with Implanted Catheter and Electrodes Allowing for Repeated Measurements Elena E. Foditsch^1,2, Karin Roider^1,2, Andrea M. Sartori^3,4,5, Thomas M. Kessler⁵, Sabik Raj Kayastha⁶, Ludwig Aigner², Marc P. Schneider^{7 1}Department of Urology, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, ²Institute of Molecular Regenerative Medicine, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, ³Brain Research Institute, University of Zürich, ⁴Department of Health Sciences and Technology, Swiss Federal Institute of Technology Zürich, ⁵Neuro-Urology, Spinal Cord Injury Center & Research, University of Zürich, Balgrist University Hospital, ⁶Department of Orthopaedics and Traumatology, Dhulikhel Hospital, Kathmandu University Hospital, ⁷Department of Urology, Inselspital, Bern University Hospital This protocol first describes the surgical procedure of the permanent implantation of a urinary bladder catheter combined with external urethral sphincter electrodes, and second, the measurement of the function of the urinary bladder and external urethral sphincter in implanted awake animals.

• Medicine

  
  Discovering Middle Ear Anatomy by Transcanal Endoscopic Ear Surgery: A Dissection Manual Lukas Anschuetz^1,2, Livio Presutti², Daniele Marchioni³, Marco Bonali², Wilhelm Wimmer^1,4, Domenico Villari², Marco Caversaccio^{1,4 1}Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Modena, ³Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Verona, ⁴Artificial Hearing Research, Artorg Center for Biomedical Engineering, University of Bern The aim of this article is to describe the methodology of exclusively endoscopic cadaveric middle ear dissection. Moreover, we aim to provide a comprehensive guide to endoscopic middle ear anatomy.

• Medicine

  
  In Vivo Imaging of Cx3cr1^gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy Despina Kokona¹, Joël Jovanovic¹, Andreas Ebneter¹, Martin S. Zinkernagel^{1 1}Department of Ophthalmology and Department of Clinical Research, Bern University Hospital and University of Bern This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively.

• Neuroscience

  
  Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique Edin Nevzati^1,2, Jeannine Rey², Daniel Coluccia^1,2, Donato D'Alonzo^1,2, Basil Grüter^1,2, Luca Remonda³, Javier Fandino^1,2, Serge Marbacher^{1,2 1}Department of Neurosurgery, Kantonsspital Aarau, ²Neuro Lab, Research Group for Experimental Neurosurgery and Neurocritical Care, Department of Intensive Care Medicine, University Hospital and University of Bern, ³Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau Reproducable experimental animal models are needed for the testing of novel embolization materials, which have been designed to treat endovascular occlusion of intracranial aneurysms (IA). The present study aims to develop a safe and standardized surgical technique for stent assisted embolization of saccular aneurysms in a rat animal model.

• Medicine

  
  The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects Lukas Andereggen^3,4,5, Volker Neuschmelting^1,6, Michael von Gunten⁷, Hans Rudolf Widmer⁵, Jukka Takala¹, Stephan M. Jakob¹, Javier Fandino^1,2, Serge Marbacher^{1,2 1}Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), ²Department of Neurosurgery, Kantonsspital Aarau, ³ The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.