University of Bern

35 articles published in JoVE

• Behavior

  
  Estimating Vestibular Perceptual Thresholds Using a Six-Degree-Of-Freedom Motion Platform Matthias Ertl¹, Daniel C. Fitze¹, Gerda Wyssen¹, Fred W. Mast^{1 1}Institute of Psychology, University of Bern In this article, we describe the methods, procedures, and technologies required to estimate vestibular perceptual thresholds using a six-degree-of-freedom motion platform.

• Medicine

  
  Robotic Cochlear Implantation for Direct Cochlear Access Marco Caversaccio^1,2, Georgios Mantokoudis¹, Franca Wagner³, Philipp Aebischer^1,2, Stefan Weder^1,2, Wilhelm Wimmer^{1,2 1}Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern, ³Department of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital Robotic cochlear implantation is a procedure for minimally invasive inner ear access. Compared to conventional surgery, robotic cochlear implantation involves additional steps that need to be carried out in the operating room. In this article we give a description of the procedure and highlight the important aspects of robotic cochlear implantation.

• Neuroscience

  
  Using a Cell-Tracer Injection to Investigate the Origin of Neointima-Forming Cells in a Rat Saccular Side Wall Model Stefan Wanderer*^1,2, Basil E. Grüter*^1,2, Jeannine Kümin^1,2, Gwendoline Boillat^1,2, Sivani Sivanrupan², Kristina Catalano^1,2, Michael von Gunten³, Hans Rudolf Widmer⁴, Serge Marbacher^1,2,5, Lukas Andereggen^{1,2,5 1}Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Institute of Pathology Laenggasse, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Faculty of Medicine, University of Bern We performed a one-point, lipophilic cell-tracer injection to track endothelial cells, followed by an arteriotomy and suturing of sidewall aneurysms on the abdominal rat aorta. Neointima formation seemed dependent on the parent artery in decellularized aneurysms and was promoted by the recruitment from aneurysm wall cells in vital cell-rich walls.

• Medicine

  
  Performing Intracochlear Electrocochleography During Cochlear Implantation Klaus Schuerch^1,2, Manuel Waser¹, Georgios Mantokoudis¹, Lukas Anschuetz¹, Wilhelm Wimmer^1,2, Marco Caversaccio^1,2, Stefan Weder^{1 1}Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern Electrocochleography (ECochG) measures inner ear potentials generated in response to acoustic stimulation. In cochlear implant (CI) candidates, such inner ear potentials can be measured directly with the implant electrodes. In this video, we systematically explain how to perform ECochG recordings during CI surgery.

• Medicine

  
  The Microscopic Transcanal Approach in Stapes Surgery Revisited Georgios Mantokoudis¹, Stefan Weder¹, Lukas Anschuetz¹, Sandra Gunser¹, Urs Borner¹, Marco D. Caversaccio^{1 1}Department of Otorhinolaryngology, Head and Neck Surgery, lnselspital, Bern University Hospital, University of Bern This article describes the microscopic transcanal technique for stapes surgery, providing step-by-step surgical instructions for familiarizing surgeons with this approach.

• Biology

  
  5-Ethynyl-2'-Deoxyuridine/Phospho-Histone H3 Dual-Labeling Protocol for Cell Cycle Progression Analysis in Drosophila Neural Stem Cells Rohan Chippalkatti^1,2, Beat Suter^{1 1}Cell Biology, University of Bern, ²Department of life sciences and medicine, University of Luxembourg Cell cycle analysis with 5-ethynyl-2'-deoxyuridine (EdU) and phospho-histone H3 (pH3) labeling is a multi-step procedure that may require extensive optimization. Here, we present a detailed protocol that describes all steps for this procedure including image analysis and quantification to distinguish cells in different cell cycle phases.

• Medicine

  
  Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit Gwendoline Boillat^1,2, Tim Franssen², Basil Grüter^1,2, Stefan Wanderer^1,2, Kristina Catalano², Daniela Casoni³, Lukas Andereggen^1,2, Serge Marbacher^{1,2 1}Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Experimental Surgery Facility, Department for Biomedical Research, Faculty of Medicine, University of Bern This protocol describes the steps for the creation of a rabbit model with two elastase-digested aneurysms with different hemodynamics (stump and bifurcation constellation). This allows the testing of novel endovascular devices in aneurysms with different angioarchitecture and hemodynamic conditions within a single animal.

• Neuroscience

  
  Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit Stefan Wanderer^1,2, Claudia Waltenspuel², Basil E. Grüter^1,2, Fabio Strange^1,2, Sivani Sivanrupan², Luca Remonda³, Hans Rudolf Widmer⁴, Daniela Casoni⁵, Lukas Andereggen^1,2, Javier Fandino^1,2, Serge Marbacher^{1,2 1}Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, ³Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, ⁴Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, ⁵Department for Biomedical Research, Faculty of Medicine, University of Bern Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits.

• Neuroscience

  
  Muscle Velocity Recovery Cycles to Examine Muscle Membrane Properties Agnes Witt¹, Hugh Bostock², Werner J. Z'Graggen³, S. Veronica Tan⁴, Alexander Gramm Kristensen¹, Rikke Søgaard Kristensen¹, Lotte Hardbo Larsen¹, Zennia Zeppelin¹, Hatice Tankisi^{1 1}Department of Clinical Neurophysiology, Aarhus University Hospital, ²UCL Queen Square Institute of Neurology, Queen Square House, ³Departments of Neurology and Neurosurgery, Inselspital, Bern University Hospital, University of Bern, ⁴MRC Centre for Neuromuscular Diseases, UCL Institute of Neurology, The National Hospital for Neurology and Neurosurgery Presented here is a protocol for the recording of muscle velocity recovery cycles (MVRCs), a new method of examining muscle membrane properties. MVRCs enable in vivo assessment of muscle membrane potential and alterations in muscle ion channel function in relation to pathology, and it enables the demonstration of muscle depolarization in neurogenic muscles.

• Neuroscience

  
  Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models Fabio Strange^1,2, Sivani Sivanrupan², Basil E. Gruter^1,2, Jeannine Rey², Dominik Taeschler², Javier Fandino^1,2, Serge Marbacher^{1,2 1}Department of Neurosurgery, Kantonsspital Aarau, ²Cerebrovascular Research Group, Department for BioMedical Research (DBMR), University of Bern We present a protocol to efficiently evaluate aneurysm perfusion and vessel patency of sidewall aneurysm in rats and rabbits, using fluorescein-based fluorescence video angiography (FVA). With a positive predictive value of 92.6%, it is a simple but very effective and economical method with no special equipment required.

• Neuroscience

  
  Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay Elena Vacchi¹, Sandra Pinton¹, Alain Kaelin-Lang^1,2,3,4, Giorgia Melli^{1,2 1}Laboratory for Biomedical Neurosciences, Neurocenter of Southern Switzerland, ²Neurology Department, Neurocenter of Southern Switzerland, ³Department of Neurology, Inselspital, Bern University Hospital, University of Bern, ⁴Faculty of Biomedical Sciences, Università della Svizzera Italiana Here, we present a protocol for a free-floating indirect immunofluorescence assay on skin biopsy sections that allows for the identification of disease specific conformation variants of alpha synuclein involved in Parkinson disease and multiple proteins of the peripheral nervous system.

• Immunology and Infection

  
  Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo Silvia M. Tietz¹, Britta Engelhardt^{1 1}Theodor Kocher Institute, University of Bern Here, we present protocols to investigate impairment of the neurovascular unit during experimental autoimmune encephalomyelitis in vivo. We specifically address how to determine blood-brain barrier permeability and gelatinase activity involved in leukocyte migration across the glia limitans. 

• Environment

  
  Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR Pamela Nicholson¹, Pattarasuda Rawiwan², Win Surachetpong^{2 1}Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, ²Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University This protocol diagnoses Tilapia Lake Virus (TiLV) in tilapia tissues using RT-PCR methodologies. The entire method is described from tissue dissection to total RNA extraction, followed by cDNA synthesis and detection of TiLV by either conventional PCR or quantitative PCR using dsDNA binding a fluorescent binding dye.

• Immunology and Infection

  
  Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy Miguel Palomino-Segura*^1,2, Tommaso Virgilio*^1,2, Diego Morone¹, Diego U. Pizzagalli^1,3, Santiago F. Gonzalez^{1 1}Faculty of Biomedical Sciences, Institute for Research in Biomedicine, Università della Svizzera italiana (USI), ²Graduate School of Cellular and Molecular Sciences, Faculty of Medicine, University of Bern, ³Institute of Computational Science, Università della Svizzera italiana (USI) In this study, we present a protocol to perform two-photon intravital imaging and cell interaction analysis in the murine tracheal mucosa after infection with influenza virus. This protocol will be relevant for researchers studying immune cell dynamics during respiratory infections.

• Immunology and Infection

  
  Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy Xenia Ficht*¹, Flavian Thelen*¹, Bettina Stolp*^1,2, Jens V. Stein^{1 1}Theodor-Kocher Institute, University of Bern, ²Center for Infectious Diseases, Integrative Virology, University Clinic of Heidelberg We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.

• Environment

  
  On the Preparation and Testing of Fuel Cell Catalysts Using the Thin Film Rotating Disk Electrode Method Masanori Inaba¹, Jonathan Quinson¹, Jan Rudolf Bucher², Matthias Arenz^{1,2 1}Nano-Science Center, Department of Chemistry, University of Copenhagen, ²Department of Chemistry and Biochemistry, University of Bern Preparing and testing Pt/C fuel cell catalysts is subject to continuous discussion in the scientific community with respect to reproducibility and best practice. With the presented work, we intend to present a step-by-step tutorial to make and test Pt/C catalysts, which can serve as benchmark for novel catalyst systems.

• Medicine

  
  Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures Mai M. Abdelhafez^1,2, Jane Shaw¹, Jonas Wilbs³, Alain Despont¹, Robert Rieben^{1 1}Department for Biomedical Research, University of Bern, ²Graduate School for Cellular and Biomedical Sciences, University of Bern, ³Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL) Here we demonstrate a protocol to standardize sampling procedures of an established porcine model of acute myocardial infarction in order to increase its translational value in the understanding of the pathophysiology of myocardial ischemia/reperfusion injury and to test novel drug candidates.

• Neuroscience

  
  Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish Federica M. Conedera^1,2,3, Petra Arendt¹, Carolyn Trepp^1,2,3, Markus Tschopp¹, Volker Enzmann^{1,2 1}Department of Ophthalmology, University Hospital of Bern, University of Bern, ²Department of Clinical Research, University of Bern, ³Graduate School for Cellular and Biomedical Sciences, University of Bern The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

• Medicine

  
  Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood Riccardo Sfriso^1,2, Anjan Bongoni³, Yara Banz⁴, Nikolai Klymiuk⁵, Eckhard Wolf⁵, Robert Rieben^{1 1}Department of Clinical Research, University of Bern, ²Graduate School for Cellular and Biomedical Sciences, University of Bern, ³ We present an in vitro model which allows the study and analysis of coagulation in whole, non-anticoagulated blood. Anticoagulation in the system depends on the natural anticoagulation effect of healthy endothelial cells and endothelial cell activation will result in clotting.

• Biology

  
  High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells Siamak Djafarzadeh¹, Stephan M. Jakob^{1 1}Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern High-resolution respirometry is used to determine mitochondrial oxygen consumption. This is a straightforward technique to determine mitochondrial respiratory chain complexes' (I-IV) respiratory rates, maximal mitochondrial electron transport system capacity, and mitochondrial outer membrane integrity.

• Biology

  
  Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments Maria C. Maldifassi*¹, Nisa Wongsamitkul*¹, Roland Baur¹, Erwin Sigel^{1 1}Institute of Biochemistry and Molecular Medicine, University of Bern Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.

• Neuroscience

  
  Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays Stefan Hahnewald^1,2, Marta Roccio^1,2, Anne Tscherter³, Jürg Streit³, Ranjeeta Ambett^1,2, Pascal Senn^{1,2,4 1}Department of Otorhinolaryngology, Inselspital and University of Bern, ²Department of Clinical Research, Inselspital and University of Bern, ³Department of Physiology, University of Bern, ⁴Department of Clinical Neurosciences, Service ORL & HNS, University Hospital Geneva We present a protocol to culture primary murine spiral ganglion neuron explants on multi electrode arrays to study neuronal response profiles and optimize stimulation parameters. Such studies aim to improve the neuron-electrode interface of cochlear implants to benefit hearing in patients as well as the energy consumption of the device.

• Behavior

  
  Concurrent Electroencephalography Recording During Transcranial Alternating Current Stimulation (tACS) Kristoffer D. Fehér¹, Yosuke Morishima^{1,2 1}Division of Systems Neuroscience of Psychopathology, Translational Research Center, University Hospital of Psychiatry, University of Bern, ²Japan Science and Technology Agency, PRESTO In this article we explain how to set up a concurrent transcranial alternating current stimulation and EEG experiment.

• Neuroscience

  
  Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays Martina Heidemann¹, Jürg Streit¹, Anne Tscherter^{1 1}Department of Physiology, University of Bern Here we present a protocol that is based on spinal cord slices cultured on multi-electrode arrays to study functional regeneration of propriospinal connections in vitro.

• Biology

  
  Bone Conditioned Medium: Preparation and Bioassay Jordi Caballé-Serrano^1,2,3, Kosaku Sawada^2,5, Guenther Schuldt Filho^1,2,6, Dieter D. Bosshardt^1,4, Daniel Buser¹, Reinhard Gruber^{1,2 1}Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, ²Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, ³Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Universitat Internacional de Catalunya, ⁴Robert K. Schenk Laboratory of Oral Histology, School of Dental Medicine, University of Bern, ⁵Department of Cranio Maxillofacial Surgery, Inselspital, University of Bern, ⁶Department of Implant Dentistry, School of Dentistry, Universidade Federal de Santa Catarina We describe here how to prepare bone-conditioned medium (BCM) and test its activity in vitro.

• Biology

  
  Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics Ellinor Maurer¹, Markus Tschopp^1,2, Christoph Tappeiner¹, Pauline Sallin³, Anna Jazwinska³, Volker Enzmann^{1 1}Department of Ophthalmology, Inselspital, University of Bern, ²Department of Ophthalmology, University Hospital of Basel, ³Department of Biology, University of Fribourg Herein we demonstrate quantification of retinal de- and regeneration and its impact on visual function using N-methyl-N-nitrosourea in the adult zebrafish. Loss of visual acuity and decreased photoreceptor numbers were followed by proliferation in the inner nuclear layer. Complete morphological and functional regeneration occurred 30 days after the initial treatment.

• Medicine

  
  The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects Lukas Andereggen^3,4,5, Volker Neuschmelting^1,6, Michael von Gunten⁷, Hans Rudolf Widmer⁵, Jukka Takala¹, Stephan M. Jakob¹, Javier Fandino^1,2, Serge Marbacher^{1,2 1}Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), ²Department of Neurosurgery, Kantonsspital Aarau, ³ The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

• Medicine

  
  A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies Inti Zlobec¹, Guido Suter¹, Aurel Perren¹, Alessandro Lugli^{1 1}Institute of Pathology, University of Bern The protocol aims at optimizing the construction and quality of tissue microarrays for biomarker research. It includes aspects of planning and design, digital pathology, virtual slide annotation, and automated tissue arraying.

• Chemistry

  
  Nucleoside Triphosphates - From Synthesis to Biochemical Characterization Marcel Hollenstein¹, Christine Catherine Smith¹, Michael Räz^{1 1}Department of Chemistry and Biochemistry, University of Bern The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.

• Immunology and Infection

  
  Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus Christina Stoeckle^1,2, Ioanna A. Rota¹, Eva Tolosa³, Christoph Haller⁴, Arthur Melms⁵, Eleni Adamopoulou^{1 1}Department of General Neurology, Hertie Institute for Clinical Brain Research, ²Institute of Pharmacology, University of Bern, ³Department of Immunology, University Medical Center Hamburg-Eppendorf, ⁴Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, ⁵Department of Neurology, University Hospital Erlangen This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.

• Medicine

  
  Bioluminescent Orthotopic Model of Pancreatic Cancer Progression Ming G. Chai¹, Corina Kim-Fuchs^1,2, Eliane Angst², Erica K. Sloan^{1,3 1}Monash Institute of Pharmaceutical Sciences, Monash University, ²Department of Visceral Surgery and Medicine, University of Bern, ³Cousins Center for Neuroimmunology, University of California Los Angeles Improved understanding of pancreatic cancer biology is critically needed to enable the development of better therapeutic options to treat pancreatic cancer. To address this need, we demonstrate an orthotopic model of pancreatic cancer that permits non-invasive monitoring of cancer progression using in vivo bioluminescence imaging.

• Bioengineering

  
  Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model Stefanie Grafmüller^1,2,3, Pius Manser², Harald F. Krug², Peter Wick², Ursula von Mandach^{1 1}Department of Obstetrics, Perinatal Pharmacology, University Hospital Zurich, ²Laboratory for Materials - Biology Interactions, EMPA Swiss Federal Laboratories for Materials Testing and Research, ³Graduate School for Cellular and Biomedical Sciences, University of Bern The ex vivo dual recirculating human placental perfusion model can be used to investigate the transfer of xenobiotics and nanoparticles across the human placenta. In this video protocol we describe the equipment and techniques required for a successful execution of a placenta perfusion.

• Immunology and Infection

  
  Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test Thomas Bodmer¹, Angelika Ströhle^{2 1}Institute for Infectious Diseases, University of Bern, ²MCL Laboratories Inc. The Xpert MTB/RIF test integrates sample decontamination, hands-free operation, on-board sample processing, and ultra-sensitive hemi-nested PCR for the simultaneous detection of Mycobacterium tuberculosis and rifampicin resistance, either in expectorated sputum or concentrated sputum sediments, in approximately two hours. Testing is standardized and requires only moderate laboratory infrastructure and training.

• Bioengineering

  
  Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture Samantha C.W. Chan¹, Benjamin Gantenbein-Ritter^{1 1}ARTORG Center for Biomedical Engineering, University of Bern This protocol illustrates a harvesting technique for coccygeal bovine intervertebral discs for organ culture for in vitro organ culture.

• Medicine

  
  Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects Camillo Sherif*^1,2,3, Javier Fandino*^2,4, Salome Erhardt^2,4, Antonio di Ieva⁵, Monika Killer⁶, Guenther Kleinpeter¹, Serge Marbacher^{2,4 1}Department of Neurosurgery, Hospital Rudolfstiftung, ²Department of Neurosurgery, Kantonsspital Aarau, ³Cerebrovascular Research Group, Department of Biomedical Research, Medical University of Vienna, ⁴Cerebrovascular Research Group, Department of Intensive Care Medicine, University of Berne, ⁵Centre for Anatomy and Cell Biology, Department of Systematic Anatomy, Medical University of Vienna, ⁶Department of Neurology, Paracelsus University Salzburg An optimized technique for the microsurgical creation of arterial bifurcation aneurysms mimicking bifurcation human cerebral aneurysms is described. A venous pouch is sutured into an artificially created true bifurcation of both common carotid arteries. Facilitated microsurgical techniques and aggressive postoperative anticoagulation and analgesia lead to minimized morbidity rates and high aneurysm patency rates.