University of Defence 1 article published in JoVE Biology Simple Detection of Primary Cilia by Immunofluorescence Alzbeta Filipova1, Daniel Diaz Garcia2, Josef Dvorak3, Stanislav Filip4, Marcela Jelicova1, Zuzana Sinkorova1 1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University Primary cilia are extracellular structures associated with the centriole. Primary cilia detection by immunofluorescent staining is a relatively simple procedure that results in extremely high-quality images. In this protocol, fibroblasts expressing primary cilia were fixed, immunostained, and imaged in a fluorescent or confocal microscope.
