2 articles published in JoVE
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone Sandra Zehentmeier1, Zoltan Cseresnyes2,3, Juan Escribano Navarro4, Raluca A. Niesner2, Anja E. Hauser1,5 1Immunodynamics, German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics, German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.
Detection of Histone Modifications in Plant Leaves Michal Jaskiewicz1,2, Christoph Peterhansel3, Uwe Conrath2 1Department of Botany, RWTH Aachen University, 2Department of Plant Physiology, RWTH Aachen University, 3Department of Botany, Leibniz University A reliable and useful approach to detect histone modifications on specific plant genes is described. The approach combines chromatin immunoprecipitation (ChIP) and real-time quantitative PCR. It allows detection of histone modifications on specific genes with a role in diverse physiological processes.