3 articles published in JoVE
The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects Lukas Andereggen3,4,5, Volker Neuschmelting1,6, Michael von Gunten7, Hans Rudolf Widmer5, Jukka Takala1, Stephan M. Jakob1, Javier Fandino1,2, Serge Marbacher1,2 1Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), 2Department of Neurosurgery, Kantonsspital Aarau, 3 The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.
Murine Model of CD40-activation of B cells Tanja M. Liebig1, Anne Fiedler1, Nela Klein-Gonzalez1, Alexander Shimabukuro-Vornhagen1, Michael von Bergwelt-Baildon1 1Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine, University Hospital of Cologne In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.
Generation of Human CD40-activated B cells Tanja M. Liebig1, Anne Fiedler1, Shahram Zoghi1, Alexander Shimabukuro-Vornhagen1, Michael S. von Bergwelt-Baildon1 1Laboratory for Tumor and Transplantation Immunology and Stem Cell Transplantation Program, University Hospital of Cologne, Department I of Internal Medicine In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.