2 articles published in JoVE
Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection Gabriella Shull*1,2, Christiane Haffner*3, Wieland B. Huttner3, Elena Taverna3,4, Suhasa B. Kodandaramaiah1,5,6 1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota This protocol demonstrates the use of a robotic platform for microinjection into single neural stem cells and neurons in brain slices. This technique is versatile and offers a method of tracking cells in tissue with high spatial resolution.
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources Adrian W. Briggs1, Jeffrey M. Good1, Richard E. Green1, Johannes Krause1, Tomislav Maricic1, Udo Stenzel1, Svante Pääbo1 1Max-Planck Institute for Evolutionary Anthropology, Leipzig We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.