2 articles published in JoVE
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue Jacek R. Wiśniewski1 1Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.
Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy Felix Spira1, Julia Dominguez-Escobar1, Nikola Müller1,2, Roland Wedlich-Söldner1 1AG Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, 2Helmholtz Zentrum München Total Internal Reflection Fluorescence (TIRF) microscopy is a powerful approach to observe structures close to the cell surface at high contrast and temporal resolution. We demonstrate how TIRF can be employed to study protein dynamics at the cortex of cell wall-enclosed bacterial and fungal cells.