University of Stuttgart

8 articles published in JoVE

• Biochemistry

  
  Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy Shuo Wang¹, Stephan Nussberger^{1 1}Biophysics Department, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart This protocol describes how to use TIRF microscopy to track individual ion channels and determine their activity in supported lipid membranes, thereby defining the interplay between lateral membrane movement and channel function. It describes how to prepare the membranes, record the data, and analyze the results.

• Medicine

  
  Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow Antonia Link¹, Giorgio Cattaneo^2,3, Eduard Brynda⁴, Tomas Riedel⁴, Johanka Kucerova⁴, Christian Schlensak¹, Hans Peter Wendel¹, Stefanie Krajewski¹, Tatjana Michel^{1 1}Department of Thoracic, Cardiac and Vascular Surgery, University Hospital Tuebingen, ²Acandis GmbH, ³Institute of Biomedical Engineering, University of Stuttgart, ⁴Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic This protocol describes a comprehensive hemocompatibility evaluation of blood-contacting devices using laser-cut neurovascular implants. A flow loop model with fresh, heparinized human blood is applied to mimic blood flow. After perfusion, various hematologic markers are analyzed and compared to the values gained directly after blood collection for hemocompatibility evaluation of the tested devices.

• Chemistry

  
  Synthesis of Soft Polysiloxane-urea Elastomers for Intraocular Lens Application Natascha Riehle*^1,2, Sibylle Thude*³, Andreas Kandelbauer^1,2, Günter E. M. Tovar^3,4, Günter Lorenz^{1,2 1}Reutlingen Research Institute, Reutlingen University, ²School of Applied Chemistry, Reutlingen University, ³Fraunhofer-Institute for Interfacial Engineering and Biotechnology IGB, ⁴Institute of Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart This study describes synthetic routes for aminopropyl-terminated polydimethylsiloxanes and polydimethyl-methyl-phenyl-siloxane-block copolymers and for soft polysiloxane-based urea (PSU) elastomers. It presents the application of PSUs as accommodating an intraocular lens. An evaluation method for in vitro cytotoxicity is also described.

• Engineering

  
  A 100 KW Class Applied-field Magnetoplasmadynamic Thruster Baojun Wang¹, Haibin Tang², Yibai Wang¹, Chao Lu¹, Cheng Zhou³, Yangyang Dong¹, Ge Wang³, Yuntian Cong³, Daniel Luu⁴, Jinbin Cao^{2 1}Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Astronautics, Beihang University, ²Key Laboratory of Spacecraft Design Optimization & Dynamic Simulation Technologies of Ministry of Education, School of Space and Environment, Beihang University, ³Beijng Institute of Control Engineering, ⁴School of Aerospace Engineering and Geodesy, University of Stuttgart The goal of this protocol is to introduce the design of a 100 kW class applied-field magnetoplasmadynamic thruster and relevant experimental methods.

• Environment

  
  Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method Eduard Rott¹, Tobias Reinhardt¹, Stephan Wasielewski¹, Ellen Raith-Bausch¹, Ralf Minke^{1 1}Institute for Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart This paper introduces a procedure to investigate the adsorption of phosphonates onto iron-containing filter materials, particularly granular ferric hydroxide, with little effort and high reliability. In a buffered solution, the phosphonate is brought into contact with the adsorbent using a rotator and then analyzed via a miniaturized phosphorus determination method.

• Engineering

  
  How to Ignite an Atmospheric Pressure Microwave Plasma Torch without Any Additional Igniters Martina Leins¹, Sandra Gaiser¹, Andreas Schulz¹, Matthias Walker¹, Uwe Schumacher¹, Thomas Hirth^{1 1}Institute of Interfacial Process Engineering and Plasma Technology, University of Stuttgart This movie shows how an atmospheric plasma torch can be ignited by microwaves with no additional igniters and provides a stable and continuous plasma operation suitable for plenty of applications.

• Biology

  
  Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays Srikanth Kudithipudi¹, Denis Kusevic¹, Sara Weirich¹, Albert Jeltsch^{1 1}Institute of Biochemistry, Stuttgart University Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

• Bioengineering

  
  Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy Miriam Votteler^1,2, Daniel A. Carvajal Berrio², Marieke Pudlas^2,3, Heike Walles^2,4, Katja Schenke-Layland^{1,2 1}Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST), Eberhard Karls University, Tübingen, ²Department of Cell and Tissue Engineering, Fraunhofer Institute of Interfacial Engineering and Biotechnology (IGB) Stuttgart, Germany, ³Department for Medical Interfacial Engineering (IGVT), University of Stuttgart, Germany, ⁴Institute of Tissue Engineering and Regenerative Medicine, Julius-Maximillians University, Würzburg, Germany Raman spectroscopy is a suitable technique for the non-contact, label-free analysis of living cells, tissue-engineered constructs and native tissues. Source-specific spectral fingerprints can be generated and analyzed using multivariate analysis.