3 articles published in JoVE
A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC) Katharina Grosser1,2, Nicole M. van Dam1,2 1German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 2Institute of Ecology, Friedrich Schiller University Jena Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.
Whole Mount Labeling of Cilia in the Main Olfactory System of Mice Sonja Oberland1,2, Eva Maria Neuhaus1,2 1Pharmacology and Toxicology, Universitaetsklinikum Jena, 2Charite-Universitaetsmedizin Berlin Cilia of olfactory sensory neurons contain proteins of the signal transduction cascade, but a detailed spatial analysis of their distribution is difficult in cryosections. We describe here an optimized approach for whole mount labeling and en face visualization of ciliary proteins.
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation Jiankai Luo1, Xin Yan1, Juntang Lin2, Arndt Rolfs1 1Albrecht-Kossel-Institute for Neuroregeneration, School of Medicine University of Rostock, 2Institute of Anatomy I, School of Medicine University of Jena A method of gene transfer into chicken embryos at later incubation stages (older than Hamburger and Hamilton stage (HH) 22) is described. This method overcomes disadvantages of in ovo electroporation applied to older chicken embryos and is a useful technique to study gene function and regulation at older developmental stages.