2 articles published in JoVE
Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects Yoko Miura1,2,3, Joachim Pruessner1, Carla Lotta Mertineit1, Katharina Kern1, Michael Muenter1, Moritz Moltmann2, Veit Danicke2, Ralf Brinkmann1,2 1Institute of Biomedical Optics, University of Luebeck, 2Medical Laser Center Luebeck GmbH, University of Leubeck, 3Department of Ophthalmology, University of Luebeck An original experimental setup for heating cells in a culture dish using 1.94 µm continuous-wave laser radiation is introduced here. Using this method, the biological responses of retinal pigment epithelial (RPE) cells after different thermal exposures can be investigated.
A Human Fallopian Tube Model for Investigation of C. trachomatis Infections Stefan Jerchel1, Gudrun Knebel2, Peter König2, Michael K. Bohlmann3, Jan Rupp1,4 1Institute of Medical Microbiology and Hygiene, University of Lübeck, 2Institute of Anatomie, University of Lübeck, 3Department of Obstetrics and Gynecology, University Hospital of Schleswig-Holstein, University of Lübeck, 4Medical Clinic III, University Hospital of Schleswig-Holstein, University of Lübeck We describe an ex vivo infection model for visualisation of direct interactions from bacterial pathogens with human fallopian tube cells. The whole organ tissue model was established to investigate C. trachomatis induced pathology to the female fallopian tube under "life-like" conditions.