Hannover Medical School

11 articles published in JoVE

• Medicine

  
  Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model Oliver Beetz*¹, Felix Oldhafer*¹, Clara A. Weigle¹, Sebastian Cammann¹, Daphne DeTemple¹, Lion Sieg², Hendrik Eismann², Thomas Palmaers², Florian W. R. Vondran^{1 1}Department of General, Visceral and Transplant Surgery, Hannover Medical School, ²Department of Anaesthesiology and Intensive Care Medicine, Hannover Medical School In this protocol, a model of porcine orthotopic liver transplantation after static cold storage of donor organs for 20 h without the use of a veno-venous bypass during engraftment is described. The approach uses a simplified surgical technique with minimization of the anhepatic phase and sophisticated volume and vasopressor management.

• Immunology and Infection

  
  A Modified Surgical Technique for Kidney Transplantation in Mice Decheng Yin*^1,2, Jian Fu*^1,3, Rongjun Chen⁴, Nelli Shushakova⁵, Ida Allabauer¹, Xin-Yi Wei¹, Mario Schiffer⁶, Diana Dudziak⁷, Song Rong*⁵, André Hoerning*^{1 1}Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women’s and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Department of Pediatric Kidney, Liver, and Metabolic Diseases, Hannover Medical School, ⁵Department of Nephrology, Hannover Medical School, ⁶Department of Nephrology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ⁷Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.

• Medicine

  
  Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model Decheng Yin*^1,2, Jian Fu*^1,3, Ida Allabauer¹, Oliver Witzke⁴, Song Rong⁵, André Hoerning^{1 1}Department of Pediatric and Adolescent Medicine, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nürnberg, ²Department of Pediatric Surgery, Chengdu Women's and Children's Central Hospital, ³The Key Laboratory of Hainan Trauma and Disaster Rescue, The first affiliated Hospital of Hainan Medical University, ⁴Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, ⁵Department of Nephrology, Hannover Medical School A novel technique for blood circuit reconstruction in a heterotopic abdominal mouse heart transplantation model is demonstrated.

• Medicine

  
  Implantation of Combined Telemetric ECG and Blood Pressure Transmitters to Determine Spontaneous Baroreflex Sensitivity in Conscious Mice René D. Rötzer¹, Verena F. Brox¹, Konstantin Hennis¹, Stefan B. Thalhammer¹, Martin Biel^1,2, Christian Wahl-Schott³, Stefanie Fenske^{1,2 1}Center for Integrated Protein Science (CIPS-M) and Center for Drug Research, Department of Pharmacy, Ludwig-Maximilians-Universität München, ²German Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, ³Hannover Medical School, Institute for Neurophysiology The baroreflex is a heart-rate regulation mechanism by the autonomic nervous system in response to blood-pressure changes. We describe a surgical technique to implant telemetry transmitters for continuous and simultaneous measurement of electrocardiogram and blood pressure in mice. This can determine spontaneous baroreflex sensitivity, an important prognostic marker for cardiovascular disease.

• Immunology and Infection

  
  An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats Clara A. Weigle¹, Thorsten Lieke¹, Florian W. R. Vondran¹, Kai Timrott¹, Jürgen Klempnauer¹, Oliver Beetz^{1 1}Regenerative Medicine and Experimental Surgery, Department of General, Visceral and Transplant Surgery, Hannover Medical School We describe a model of heterotopic abdominal heart transplantation in rats, implying modifications of current strategies, which lead to a simplified surgical approach. Additionally, we describe a novel rejection model by in-ear injection of vital cardiac muscle cells, allowing further transplant immunological analyses in rats.

• Medicine

  
  Veno-Venous Extracorporeal Membrane Oxygenation in a Mouse Nodir Madrahimov¹, Abdurasul Khalikov¹, Erin C. Boyle¹, Ruslan Natanov¹, Ann-Kathrin Knoefel¹, Thierry Siemeni¹, Klaus Hoeffler¹, Axel Haverich¹, Ulrich Maus*², Christian Kuehn*^{1 1}Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, ²Department of Pneumology, Hannover Medical School Here we present a protocol describing the technique of veno-venous extracorporeal membrane oxygenation (ECMO) in a non-intubated, spontaneously breathing mouse. This murine model of ECMO can be effectively implemented in experimental studies of acute and end-stage lung diseases.

• Medicine

  
  Cardiopulmonary Bypass in a Mouse Model: A Novel Approach Nodir Madrahimov¹, Ruslan Natanov¹, Erin C. Boyle¹, Tobias Goecke¹, Ann-Kathrin Knöfel¹, Valentyna Irkha¹, Anna Solovieva², Klaus Höffler¹, Ulrich Maus³, Christian Kühn¹, Issam Ismail¹, Gregor Warnecke¹, Malakh-Lal Shrestha¹, Serghei Cebotari*¹, Axel Haverich*^{1 1}Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, ²Department of Hematology, Oncology, Immunology, Rheumatology, and Pulmonology, University Hospital Tuebingen, ³Department of Pneumology, Hannover Medical School This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage.

• Neuroscience

  
  Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis Alexander Brinkmann*^1,2, Camille Okom*^1,2, Eugen Kludt^1,2,4, Detlev Schild^{1,2,3 1}Institute of Neurophysiology and Cellular Biophysics, Georg-August-Universität Göttingen, ²Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Georg-August-Universität Göttingen, ³DFG Excellence Cluster 171, Georg-August-Universität Göttingen, ⁴German Hearing Center Hannover Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.

• Developmental Biology

  
  A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells Claudia Davenport¹, Ulf Diekmann¹, Ortwin Naujok^{1 1}Institute of Clinical Biochemistry, Hannover Medical School Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations.

• Developmental Biology

  
  Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis Verónica A. Lombardo*^1,2,3, Cécile Otten*^1,2, Salim Abdelilah-Seyfried^{1,2,3 1}Max Delbrück Center for Molecular Medicine, ²Institute of Biochemistry and Biology, University of Potsdam, ³Institute of Molecular Biology, Medizinische Hochschule Hannover To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

• Medicine

  
  Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens Dorothée Sturm^1,2, Lorella Marselli³, Florian Ehehalt^1,2, Daniela Richter¹, Marius Distler², Stephan Kersting^1,2, Robert Grützmann², Krister Bokvist⁴, Philippe Froguel⁵, Robin Liechti⁶, Anne Jörns⁷, Paolo Meda⁸, Gustavo Bruno Baretton⁹, Hans-Detlev Saeger², Anke M. Schulte¹⁰, Piero Marchetti³, Michele Solimena^{1 1}Molecular Diabetology, Paul Langerhans Institute Dresden, ²Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, ³Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, ⁴Labs DC0522, Lilly Corporate Center, ⁵Genomics, Faculty of Medicine Imperial College London, ⁶Vital-IT, SIB Swiss Institute of Bioinformatics, ⁷Clinical Biochemistry, Hannover Medical School, ⁸Cell Physiology and Metabolism, Medical School, University of Geneva, ⁹Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, ¹⁰R&D DIAB Division / Translational Medicine, Sanofi-Aventis Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.