3 articles published in JoVE
Cardiopulmonary Bypass in a Mouse Model: A Novel Approach Nodir Madrahimov1, Ruslan Natanov1, Erin C. Boyle1, Tobias Goecke1, Ann-Kathrin Knöfel1, Valentyna Irkha1, Anna Solovieva2, Klaus Höffler1, Ulrich Maus3, Christian Kühn1, Issam Ismail1, Gregor Warnecke1, Malakh-Lal Shrestha1, Serghei Cebotari*1, Axel Haverich*1 1Department of Cardiothoracic, Transplantation, and Vascular Surgery, Hannover Medical School, 2Department of Hematology, Oncology, Immunology, Rheumatology, and Pulmonology, University Hospital Tuebingen, 3Department of Pneumology, Hannover Medical School This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage.
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis Verónica A. Lombardo*1,2,3, Cécile Otten*1,2, Salim Abdelilah-Seyfried1,2,3 1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens Dorothée Sturm1,2, Lorella Marselli3, Florian Ehehalt1,2, Daniela Richter1, Marius Distler2, Stephan Kersting1,2, Robert Grützmann2, Krister Bokvist4, Philippe Froguel5, Robin Liechti6, Anne Jörns7, Paolo Meda8, Gustavo Bruno Baretton9, Hans-Detlev Saeger2, Anke M. Schulte10, Piero Marchetti3, Michele Solimena1 1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.