3 articles published in JoVE
Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia Katharina Hohlbaum1,2, Bettina Bert2,3, Silke Dietze2, Rupert Palme4, Heidrun Fink2, Christa Thöne-Reineke1 1Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, 2Institute of Pharmacology and Toxicology, Department of Veterinary Medicine, Freie Universität Berlin, 3German Federal Institute for Risk Assessment (BfR), 4Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner.
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research Christopher Weidner1, Matthias Steinfath1, Elisa Wistorf1, Michael Oelgeschläger1, Marlon R. Schneider1, Gilbert Schönfelder1,2 1Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), 2Department of Clinical Pharmacology and Toxicology, Charité-Universitätsmedizin Berlin We provide a standardized protocol for the use of gene set enrichment analysis of transcriptomic data to identify an ideal mouse model for translational research. This protocol can be used with DNA microarray and RNA sequencing data and can further be extended to other omics data if data are available.
Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples Anne Mayer-Scholl1, Edoardo Pozio2, Jennifer Gayda1, Nora Thaben1, Peter Bahn1, Karsten Nöckler1 1Federal Institute for Risk Assessment, 2Istituto Superiore di Sanità The prevention of human trichinellosis in many countries worldwide is based on the laboratory examination of muscle samples from susceptible animals by methods of digestion, of which the magnetic stirrer method is considered the gold standard.