1 article published in JoVE
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction Gunnar T.R. Hischebeth1, Sascha Gravius2, Johanna K. Buhr2, Ernst Molitor1, Matthias D. Wimmer2, Achim Hoerauf1, Isabelle Bekeredjian-Ding3, Thomas M. Randau2 1Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, 2Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, 3Division of EU Cooperation/Microbiology, Paul-Ehrlich-Institute Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.