University Hospital Munster 5 articles published in JoVE Medicine Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation Tobias M. Nowacki1, Dominik Bettenworth1, Markus Brückner1, Friederike Cordes1, Frank Lenze1, Anne Becker2, Moritz Wildgruber2, Michel Eisenblätter2 1Department of Medicine B, University Hospital Münster, 2Translational Research Imaging Center, Department of Clinical Radiology, University Hospital Münster Target-specific probes represent an innovative tool for analyzing molecular mechanisms, such as protein expression in various types of disease (e.g., inflammation, infection, and tumorigenesis). In this study, we describe a quantitative three-dimensional tomographic assessment of intestinal macrophage infiltration in a murine model of colitis using F4/80-specific fluorescence-mediated tomography. Immunology and Infection Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier Andreas Schulte-Mecklenbeck1, Urvashi Bhatia1, Tilman Schneider-Hohendorf1, Nicholas Schwab1, Heinz Wiendl*1, Catharina C. Gross*1 1Department of Neurology with Institute of Translational Neurology, University Hospital Münster Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro. Immunology and Infection An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction Ivan Kuzmanov1, Alexander M. Herrmann1, Hans-Joachim Galla2, Sven G. Meuth1, Heinz Wiendl*1, Luisa Klotz*1 1Department of Neurology, University Hospital Münster, 2Institute of Biochemistry, University of Münster Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells. Medicine Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing Philipp Lenz1,2, Markus Brückner1, Steffi Ketelhut3, Jan Heidemann4, Björn Kemper*3, Dominik Bettenworth*1 1Department of Medicine B, University Hospital Münster, 2Institute of Palliative Care, University Hospital Münster, 3Biomedical Technology Center, University of Münster, 4Department of Gastroenterology, Klinikum Bielefeld Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro. Medicine Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation Markus Brückner*1, Philipp Lenz*1, Tobias M. Nowacki1, Friederike Pott1, Dirk Foell2, Dominik Bettenworth1 1Department of Medicine B, University Hospital Münster, 2 Small animal imaging techniques allow serial diagnostic examinations and therapeutic interventions in vivo. Recently, the scope of applications has significantly widened and currently includes assessment of colonic tumor development, wound healing and monitoring of inflammation. This protocol illustrates these diverse potential applications of murine endoscopy.
