2 articles published in JoVE
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip Raphaël de Wijn1,4, Kévin Rollet1,2, Vincent Olieric3, Oliver Hennig2, Nicola Thome1, Camille Noûs1, Caroline Paulus1, Bernard Lorber1, Heike Betat2, Mario Mörl2, Claude Sauter1 1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.
DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers Jörg Schnauß*1,2, Martin Glaser*1,2, Jessica S. Lorenz1, Carsten Schuldt1,2, Christin Möser1, Martin Sajfutdinow1, Tina Händler1,2, Josef A. Käs2, David M. Smith1 1Fraunhofer Institute for Cell Therapy and Immunology, 2Institute of Experimental Physics I, Universität Leipzig Semiflexible polymers display unique mechanical properties that are extensively applied by living systems. However, systematic studies on biopolymers are limited since properties such as polymer rigidity are inaccessible. This manuscript describes how this limitation is circumvented by programmable DNA nanotubes, enabling experimental studies on the impact of filament rigidity.