German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association 2 articles published in JoVE Neuroscience Modeling Amyloid-β42 Toxicity and Neurodegeneration in Adult Zebrafish Brain Prabesh Bhattarai1, Alvin Kuriakose Thomas2, Mehmet Ilyas Cosacak1, Christos Papadimitriou1, Violeta Mashkaryan3, Yixin Zhang2, Caghan Kizil1,3 1German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association, 2B CUBE, Center for Molecular Bioengineering, Technische Universität Dresden, 3Center for Regenerative Therapies Dresden (CRTD), TU Dresden This protocol describes the synthesis, characterization, and injection of monomeric amyloid-β42 peptides for generating amyloid toxicity in adult zebrafish to establish an Alzheimer's disease model, followed by histological analyses and detection of aggregations. Neuroscience One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice Tara L. Walker1, Gerd Kempermann1,2 1Center for Regenerative Therapies Dresden, Technische Universität Dresden, 2German Center for Neurodegenerative Diseases (DZNE) Dresden Here we describe a detailed protocol for the simultaneous generation of neural precursor cell cultures, as either adherent monolayers or neurospheres, from the subventricular zone and dentate gyrus of individual adult mice.
