3 articles published in JoVE
Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo Adrian Gericke1, Evgeny Goloborodko1, Norbert Pfeiffer1, Caroline Manicam1 1Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.
Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls Jeremy Lagrange1, Sabine Kossmann1,2, Klytaimnistra Kiouptsi1, Philip Wenzel1,2,3 1Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, 2Center for Cardiology, University Medical Center Mainz, 3German Center for Cardiovascular Research (DZHK) This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation J. Moritz Kaths1,2, Vinzent N. Spetzler1, Nicolas Goldaracena1, Juan Echeverri1, Kristine S. Louis1, Daniel B. Foltys3, Mari Strempel4, Paul Yip5, Rohan John5, Istvan Mucsi1, Anand Ghanekar1, Darius Bagli6,7, Lisa Robinson2, Markus Selzner1 1Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, 2Division of Nephrology, The Hospital for Sick Children, Toronto, 3Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, 4Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, 5Laboratory Medicine & Pathobiology, Toronto General Hospital, 6Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, 7Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.