2 articles published in JoVE
Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo Adrian Gericke1, Evgeny Goloborodko1, Norbert Pfeiffer1, Caroline Manicam1 1Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.
Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls Jeremy Lagrange1, Sabine Kossmann1,2, Klytaimnistra Kiouptsi1, Philip Wenzel1,2,3 1Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, 2Center for Cardiology, University Medical Center Mainz, 3German Center for Cardiovascular Research (DZHK) This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.