German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig 2 articles published in JoVE Chemistry A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC) Katharina Grosser1,2, Nicole M. van Dam1,2 1German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 2Institute of Ecology, Friedrich Schiller University Jena Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography. Biology Relating Stomatal Conductance to Leaf Functional Traits Wenzel Kröber1, Isa Plath1, Heike Heklau1, Helge Bruelheide1,2 1Institute of Biology / Geobotany and Botanical Garden, Martin-Luther-University Halle-Wittenberg, 2German Centre for Integrative Biodiversity Research Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.
