Hector Institute for Translational Brain Research (HITBR gGmbH) 1 article published in JoVE Developmental Biology Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells Olivia Krefft*1, Ammar Jabali*1, Vira Iefremova1, Philipp Koch1,2,3, Julia Ladewig1 1Institute of Reconstructive Neurobiology, University of Bonn, 2Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH) Cerebral organoids represent a new model system to investigate early human brain development in vitro. This article provides the detailed methodology to efficiently generate homogeneous dorsal forebrain-type organoids from human induced pluripotent stem cells including critical characterization and validation steps.
