2 articles published in JoVE
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes Irene U. Wacker1,2, Lisa Veith3, Waldemar Spomer2,4, Andreas Hofmann2,4, Marlene Thaler5, Stefan Hillmer6, Ulrich Gengenbach2,4, Rasmus R. Schröder1,2,3 1Cryo Electron Microscopy, Centre for Advanced Materials, Universität Heidelberg, 2Heidelberg Karlsruhe Research Partnership (HEiKA), 3Cryo Electron Microscopy, BioQuant, Universitätsklinikum Heidelberg, 4Institute for Automation and Applied Computer Science, Karlsruhe Institute of Technology (KIT), 5Carl Zeiss Microscopy GmbH, 6Electron Microscopy Core Facility, Universität Heidelberg This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.
Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography Hannah P. Menke1, Matthew G. Andrew2, Joan Vila-Comamala3, Christoph Rau3, Martin J. Blunt1, Branko Bijeljic1 1Department of Earth Science and Engineering, Imperial College London, 2Carl Zeiss X-Ray Microscopy, 3Diamond Manchester Imaging Branchline (I13-2), Diamond Lightsource Synchrotron fast tomography was used to dynamically image dissolution of limestone in the presence of CO2-saturated brine at reservoir conditions. 100 scans were taken at a 6.1 µm resolution over a period of 2 h.