Institut fur Molekulare Zellbiologie 2 articles published in JoVE Immunology and Infection Time-lapse Imaging of Mouse Macrophage Chemotaxis Esther van den Bos1, Stefan Walbaum1, Markus Horsthemke1, Anne C. Bachg1, Peter J. Hanley1 1Institut für Molekulare Zellbiologie Here we describe methods using time-lapse, phase-contrast microscopy to image mouse resident peritoneal macrophages in a chemotactic complement C5a gradient. The protocols can be extended to other immune cells. Immunology and Infection Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages Markus Horsthemke1, Janine Wilden1, Anne C. Bachg1, Peter J. Hanley1 1Institut für Molekulare Zellbiologie Here we describe methods using spinning disk confocal microscopy to image single phagocytic events by mouse resident peritoneal macrophages. The protocols can be extended to other phagocytic cells.
