2 articles published in JoVE
A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells Anja Baufeld1, Jens Vanselow1 1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN) A long-term culture model of bovine granulosa cells under serum-free conditions is described. This model allows researchers to study the effects of diverse factors and conditions as different plating densities on the characteristics of estrogen-producing bovine granulosa cells.
In Vivo Microinjection and Electroporation of Mouse Testis Marten Michaelis1, Alexander Sobczak1, Joachim M. Weitzel1 1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN) This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.