Interfaculty Institute for Biochemistry 1 article published in JoVE Bioengineering Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers Joseph D. Unsay1,2,3, Katia Cosentino1,2, Ana J. García-Sáez1,2 1Interfaculty Institute for Biochemistry, 2Max Planck Institute for Intelligent Systems, 3German Cancer Research Center We describe a protocol for preparation of supported lipid bilayers and its characterization using atomic force microscopy and force spectroscopy.