Fraunhofer IME 1 article published in JoVE Immunology and Infection Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues Julia Barthelmes*1, Nadja Tafferner*2, Jennifer Kurz2, Natasja de Bruin2, Michael J. Parnham2, Gerd Geisslinger1, Susanne Schiffmann2 1Institute of Clinical Pharmacology, Goethe University Hospital Frankfurt, 2Project Group for Translational Medicine & Pharmacology, Fraunhofer IME This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.
