Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) 4 articles published in JoVE Biology Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo Sören S. Hüttner1, Christian Hayn1, Hellen E. Ahrens1, Manuel Schmidt1, Henriette Henze1, Julia von Maltzahn1 1Leibniz Institute on Aging, Fritz-Lipmann-Institute In this protocol an in vitro culturing and functional analysis method for muscle stem cells is described, which preserves most of their interactions with their endogenous niche. Developmental Biology Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning Birgit Perner1, Danny Schnerwitzki1, Michael Graf1,3, Christoph Englert1,2 1Molecular Genetics, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), 2Faculty of Biology and Pharmacy, Friedrich Schiller University of Jena, 3Carl Zeiss Microscopy GmbH The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift. Neuroscience In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice Stefanie Fischer*1, Christian Engelmann*2, Karl-Heinz Herrmann3, Jürgen R. Reichenbach3, Otto W. Witte1, Falk Weih2, Alexandra Kretz1, Ronny Haenold2 1Hans Berger Department of Neurology, Jena University Hospital, 2Immunology, Leibniz Institute for Age Research, Fritz Lipmann Institute, Jena, 3Institute of Diagnostic and Interventional Radiology, Medical Physics Group, Jena University Hospital This video illustrates a method, using a clinical 3 T scanner, for contrast-enhanced MR imaging of the naïve mouse visual projection and for repetitive and longitudinal in vivo studies of optic nerve degeneration associated with acute optic nerve crush injury and chronic optic nerve degeneration in knock-out mice (p50KO). Neuroscience In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves Alexander Schulz1, Christian Walther2, Helen Morrison1, Reinhard Bauer3 1Leibniz Institute for Age Research, Fritz Lipmann Institute, 2Friedrich Schiller University Jena, 3Institute of Molecular Cell Biology & Center for Sepsis Control and Care (CSCC) Jena University Hospital, Friedrich Schiller University Jena Measurements of nerve conduction properties in vivo exemplify a powerful tool to characterize various animal models of neuromuscular diseases. Here, we present an easy and reliable protocol by which electrophysiological analysis on sciatic nerves of anesthetized mice can be performed.
