University of Siegen 2 articles published in JoVE Behavior Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies Stefanie Gierszewski1, Derek Baker1,2, Klaus Müller3, Jan-Marco Hütwohl3, Klaus-Dieter Kuhnert3, Klaudia Witte1 1Research Group of Ecology and Behavioral Biology, Institute of Biology, University of Siegen, 2University of Calgary, 3Institute of Real-Time Learning Systems, University of Siegen Using the novel FishSim Animation Toolchain, we present a protocol for non-invasive visual manipulation of public information in the context of mate-choice copying in sailfin mollies. FishSim Animation Toolchain provides an easy-to-use framework for the design, animation and presentation of computer-animated fish stimuli for behavioral experiments with live test fish. Genetics From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028 Anja Greule1, Songya Zhang1, Thomas Paululat2, Andreas Bechthold1 1Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology, Universität Siegen Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.
