2 articles published in JoVE
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon Tamara Durovic1,2, Jovica Ninkovic1,3,4 1Institute of Stem Cell Research, Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences, Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center, Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy) Presented here is an electroporation method for plasmid DNA delivery and ependymoglial cell labeling in the adult zebrafish telencephalon. This protocol is a quick and efficient method to visualize and trace individual ependymoglial cells and opens up new possibilities to apply electroporation to a broad range of genetic manipulations.
Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery Gemma Llovera*1,2, Stefan Roth*1,2, Nikolaus Plesnila1,2, Roland Veltkamp3,4, Arthur Liesz1,2 1Institute for Stroke and Dementia Research, University Hospital Munich, 2Munich Cluster for Systems Neurology (SyNergy), 3Department of Neurology, University Heidelberg, 4Imperial College, Charing Cross Hospital Various murine models of middle cerebral artery occlusion (MCAO) are widely used in experimental brain research. Here, we demonstrate the model of transcranial permanent distal MCAO which produces consistent cortical infarction of a size corresponding to damage imposed by the majority of human ischemic strokes.