Fraunhofer Institute for Molecular Biology and Applied Ecology 1 article published in JoVE Environment Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants Megan Garvey1, Johannes Klinger*1, Holger Klose*1, Rainer Fischer2, Ulrich Commandeur1 1Institute for Molecular Biotechnology, Bio7, RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.
