University of Mainz 1 article published in JoVE Medicine Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats Gert Blueschke*1, Gabi Hanna*2, Andrew N. Fontanella2, Gregory M. Palmer2, Alina Boico2, Hooney Min2, Mark W. Dewhirst2, David C. Irwin3, Yulin Zhao2, Thies Schroeder4 1Division of Plastic, Maxillofacial, and Oral Surgery, Duke University Medical Center, 2Department of Radiation Oncology, Duke University Medical Center, 3Department of Cardiology, University of Colorado Denver, 4Department of Physical Chemistry, University of Mainz A method is presented to measure microcirculatory blood flow velocity in pulmonary cancer metastases of the pleural surface in rats in an automated fashion, using closed-chest pulmonary intravital microscopy. This model has potential to be used as a widespread tool to perform physiologic research on pulmonary metastases in rodents.