2 articles published in JoVE
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence Rebecca Wallrafen1, Thomas Dresbach1, Julio S. Viotti1 1Institute of Anatomy and Embryology, University Medical Center Göttingen Here, we describe a quantitative approach to determining the distribution of a synaptic protein relative to a marker protein using immunofluorescence staining, confocal microscopy, and computer-based analysis.
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags Kerstin Menck1, Daniel Behme1, Mathias Pantke1, Norbert Reiling2, Claudia Binder1, Tobias Pukrop1, Florian Klemm1 1Department of Hematology and Oncology, University Medical Center Göttingen, 2Microbial Interface Biology Group, Research Center Borstel We present a simple and efficient protocol for the generation of human macrophages. Buffy coats are processed by double density gradient centrifugation and isolated monocytes are then differentiated to macrophages in Teflon-coated cell culture bags. This maximizes macrophage yields and facilitates cell harvesting for subsequent experiments.