2 articles published in JoVE
Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo Johannes P.W. Heidbuechel1,2, Christine E. Engeland1,3 1Department of Translational Oncology, German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT), 2Faculty of Biosciences, Heidelberg University, 3Department of Medical Oncology, NCT and Heidelberg University Hospital This protocol describes a detailed workflow for the generation and ex vivo characterization of oncolytic viruses for expression of immunomodulators, using measles viruses encoding bispecific T cell engagers as an example. Application and adaptation to other vector platforms and transgenes will accelerate the development of novel immunovirotherapeutics for clinical translation.
Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA Richard Gabriel1, Ina Kutschera1, Cynthia C Bartholomae1, Christof von Kalle1, Manfred Schmidt1 1Department of Translational Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ) Linear-amplification mediated (LAM)-PCR is a method developed to identify the exact positions of integrating viral vectors in the genome. The technique has evolved to be the superior method to study clonal dynamics in gene therapy patients, biosafety of novel vector technologies, T-cell diversity, cancer stem cell models, etc.