Aalen University 1 article published in JoVE Engineering Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope Thomas Bruns1, Sarah Schickinger1, Herbert Schneckenburger1 1Institute of Applied Research, Aalen University A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.