Robert-Bosch-Krankenhaus 1 article published in JoVE Medicine Intracoronary Acetylcholine Provocation Testing for Assessment of Coronary Vasomotor Disorders Peter Ong1, Anastasios Athanasiadis1, Udo Sechtem1 1Department of Cardiology, Robert-Bosch-Krankenhaus Intracoronary acetylcholine testing has been established for the assessment of epicardial coronary spasm more than 30 years ago. Recently, the focus has shifted towards the microcirculation and it has been shown that microvascular spasm can be detected using ACH-testing. This article describes the ACH-test and its implementation in daily routine.