2 articles published in JoVE
Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture Laurel R. Yohe*1,2, Paolo Devanna*3, Kalina T.J. Davies4, Joshua H.T. Potter4, Stephen J. Rossiter4, Emma C. Teeling5, Sonja C. Vernes*3,6, Liliana M. Dávalos*2,7 1Department of Geology & Geophysics, Yale University, 2Department of Ecology & Evolution, Stony Brook University, 3Neurogenetics of Vocal Communication, Max Planck Institute for Psycholinguistics, 4School of Biological and Chemical Sciences, Queen Mary University of London, 5School of Biology & Environmental Science, University College Dublin, 6Donders Institute for Brain, Cognition and Behavior, 7Consortium for Inter-Disciplinary Environmental Research, Stony Brook University This is a protocol for the optimal tissue preparation for genomic, transcriptomic, and proteomic analyses of bats caught in the wild. It includes protocols for bat capture and dissection, tissue preservation, and cell culturing of bat tissue.
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer Pelagia Deriziotis*1, Sarah A. Graham*1, Sara B. Estruch1, Simon E. Fisher1,2 1Language and Genetics Department, Max Planck Institute for Psycholinguistics, 2Donders Institute for Brain, Cognition and Behaviour Interactions between proteins are fundamental to all cellular processes. Using Bioluminescence Resonance Energy Transfer, the interaction between a pair of proteins can be monitored in live cells and in real time. Furthermore, the effects of potentially pathogenic mutations can be assessed.