Asklepios Fachkliniken Munich-Gauting 1 article published in JoVE Medicine Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling Michael Gerckens1,2,3, Hani N. Alsafadi4,5,6, Darcy E. Wagner4,5,6, Michael Lindner2,7, Gerald Burgstaller*1,2,3, Melanie Königshoff*1,2,3,8 1Comprehensive Pneumology Center, Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München, Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, 5Wallenberg Center for Molecular Medicine, Lund University, 6Stem Cell Centre, Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.
