3 articles published in JoVE
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging Anna M. Steyer1,2, Torben Ruhwedel1, Wiebke Möbius1,2 1Electron Microscopy Core Unit, Max Planck Institute of Experimental Medicine, 2Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB) Here we present a protocol for combining two sample processing techniques, high-pressure freezing and microwave-assisted sample processing, followed by minimal resin embedding for acquiring data with a focused ion beam scanning electron microscope (FIB-SEM). This is demonstrated using a mouse tibial nerve sample and Caenorhabditis elegans.
Simultaneous Transcranial Alternating Current Stimulation and Functional Magnetic Resonance Imaging Kathleen A. Williams1, Yuranny Cabral-Calderin1,3, Carsten Schmidt-Samoa1, Christiane Anne Weinrich1,2, Peter Dechent1, Melanie Wilke1,3,4 1Department of Cognitive Neurology, University Medicine Goettingen, 2Department of Neurology, University Medicine Goettingen, 3German Primate Center, Leibniz Institute for Primate Research, 4DFG Center for Nanoscale Microscopy & Molecular Physiology of the Brain (CNMPB) Transcranial alternating current stimulation (tACS) is a promising tool for noninvasive investigation of brain oscillations, though its effects are not completely understood. This article describes a safe and reliable setup for applying tACS simultaneously with functional magnetic resonance imaging, which can increase understanding oscillatory brain function and effects of tACS.
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration Anna Holubowska1,2, Chaitali Mukherjee1,2, Mayur Vadhvani1,2, Judith Stegmüller1,2 1Cellular and Molelcular Neurobiology, Max Planck Institute of Experimental Medicine, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB) Neuronal morphogenesis and migration are crucial events underlying proper brain development. Here, we describe methods to genetically manipulate cultured cerebellar granule neurons and the developing cerebellum for the assessment of morphology and migratory characteristics of neurons.