University of Mons 1 article published in JoVE Bioengineering Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues Thomas Grevesse1, Marie Versaevel1, Sylvain Gabriele1 1Laboratoire Interfaces et Fluides Complexes, Université de Mons We present a new polyacrylamide hydrogel, called hydroxy-PAAm, that allows a direct binding of ECM proteins with minimal cost or expertise. The combination of hydroxy-PAAm hydrogels with microcontact printing facilitates independent control of many cues of the natural cell microenvironment for studying cellular mechanostransduction.
