1 article published in JoVE
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure Serene M.L. Lee1,2, Celine Schelcher1, Maresa Demmel2,3, Maria Hauner3, Wolfgang E. Thasler4 1Experimental Surgical Research, Grosshadern Hospital, Munich, 2Center for Liver Cell Research, Grosshadern Hospital, Munich, 3Hepacult LLC, Regensburg, 4Department of Surgery, Grosshadern Hospital, Munich A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.