University Hospital Eppendorf

1 article published in JoVE

• Biochemistry

  
  Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins Eva Königshausen¹, Sebastian A. Potthoff¹, Raphael Haase¹, Catherine Meyer-Schwesinger², Ernest Kaufmann¹, L. Christian Rump¹, Johannes Stegbauer¹, Lorenz Sellin¹, Ivo Quack¹, Magdalena Woznowski^{1 1}Department of Nephrology, Medical Faculty, Heinrich-Heine-University, ²Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.