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1 article published in JoVE
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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
Eva Königshausen1, Sebastian A. Potthoff1, Raphael Haase1, Catherine Meyer-Schwesinger2, Ernest Kaufmann1, L. Christian Rump1, Johannes Stegbauer1, Lorenz Sellin1, Ivo Quack1, Magdalena Woznowski1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf
Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.
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