Federal Research Institute for Animal Health 1 article published in JoVE Neuroscience High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue Luca Zaeck1, Madlin Potratz1, Conrad M. Freuling1, Thomas Müller1, Stefan Finke1 1Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health Novel, immunostaining-compatible tissue clearing techniques like the ultimate 3D imaging of solvent-cleared organs allow the 3D visualization of rabies virus brain infection and its complex cellular environment. Thick, antibody-labeled brain tissue slices are made optically transparent to increase imaging depth and to enable 3D analysis by confocal laser scanning microscopy.
