Nanosystems Initiative Munich 1 article published in JoVE Bioengineering In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth Kilian Vogele1, Thomas Frank1, Lukas Gasser1, Marisa A. Goetzfried1, Mathias W. Hackl2, Stephan A. Sieber2, Friedrich C. Simmel1,3, Tobias Pirzer1 1Physics of Synthetic Systems - E14, Physics-Department and ZNN, Technische Universität München, 2Departmant of Chemistry, Center for Integrated Protein Science (CIPSM), Technische Universität München, 3Nanosystems Initiative Munich Presented here are protocols for the creation of peptide-based small unilamellar vesicles capable of growth. To facilitate in vesiculo production of the membrane peptide, these vesicles are equipped with a transcription-translation system and the peptide-encoding plasmid.
