Max-Planck Institute of Experimental Medicine 2 articles published in JoVE Neuroscience Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator Athanasios Katsalifis1, Angela Maria Casaril1, Constanze Depp2, Carlos Bas-Orth1 1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology, Heidelberg University, 2Department of Neurogenetics, Max-Planck-Institute for Experimental Medicine This article describes a protocol to determine differences in basal redox state and redox responses to acute perturbations in primary hippocampal and cortical neurons using confocal live microscopy. The protocol can be applied to other cell types and microscopes with minimal modifications. Biology Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles Naira Movsisyan1,2, Luis A. Pardo1 1AG Oncophysiology, Max-Planck Institute of Experimental Medicine, 2Göttingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB) Here we present a robust and detailed method of microtubule dynamics analysis in cells synchronized in prometaphase using live-cell spinning disk confocal microscopy and MATLAB-based image processing.
